Biochemistry and Genetics: Pretest Self-Assessment and by Wilson G.N., Schroeder B., Woo J.

By Wilson G.N., Schroeder B., Woo J.

Those "500 questions, solutions, and rationalization" books are designed to simulate the us scientific Licensing exam (USMLE) Step 1. every one is authored by way of a professional within the box and has been reviewed through scholars who've lately handed the examination. As Step 1 is changing into an increasing number of scientific in nature, whilst appropriate, scientific vignette-style questions requiring a two-step reasoning approach also are incorporated. the entire Pretest titles contain a "high-yield" truth part to augment key thoughts.

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Sack, pp 1–40. ) σ factor is a bacterial protein that can associate with and become a subunit of bacterial RNA polymerase. qxd 52 11/14/01 10:37 AM Page 52 Biochemistry and Genetics specificity of initiation on the core enzyme. In the presence of σ factor, RNA polymerase chooses the correct strand of duplex DNA for transcription and initiates transcription at the appropriate promoter region. In some bacteria, such as Bacillus subtilis, a specific σ factor is synthesized to change transcriptional selectivity and effect cellular changes like sporulation.

E. -CCA-CCT-AGG-TTC-AGG-GCC-CCT-AGG-TTC-AGG-CCA-CCC-TAG-GTT-CAG-CCC-CTA-GGT-TCA-GG— -CCC-CCT-AGG-AGG—— 36. Which of the following results is provided by northern blot analysis? a. b. c. d. e. Detects specific base pairs Detects DNA molecules Detects RNA molecules Detects proteins Determines chromosome structure 37. The hypothetical “stimulin” gene contains two exons that encode a protein of 100 amino acids. They are separated by an intron of 100 bp beginning after the codon for amino acid 10. Stimulin messenger RNA (mRNA) has 5′ and 3′ untranslated regions of 70 and 30 nucleotides, respectively.

The answer is d. (Murray, pp 452–467. Scriver, pp 3–45. Sack, pp 1–40. ) The gene that produces the deadly toxin of Corynebacterium diphtheriae comes from a lysogenic phage that grows in the bacteria. Prior to immunization, diphtheria was the primary cause of death in children. The protein toxin produced by this bacterium inhibits protein synthesis by inactivating elongation factor 2 (EF-2, or translocase). Diphtheria toxin is a single protein composed of two portions (A and B). The B portion enables the A portion to translocate across a cell membrane into the cytoplasm.

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