Biomembranes Part L: Membrane Biogenesis: Processing and by Nathan P. Kaplan, Nathan P. Colowick, Sidney Fleischer,

By Nathan P. Kaplan, Nathan P. Colowick, Sidney Fleischer, Becca Fleischer

FROM THE PREFACE: Volumes ninety six to ninety eight, elements J, ok, and L of the Biomembranes sequence, specialise in method to check membrane biogenesis, meeting, concentrating on, and recycling. This box is likely one of the very fascinating and energetic components of study. destiny volumes will take care of shipping and different elements of membrane functionality

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By use of the methods described in this section, we have (a) measured the effects of secretagogues, 3 ions, '°,'~ cyclic nucleotides, '2 and ATP stores 3 on L0G. A. Scheele and A. Haymovits, J. Biol. Chem. 254, 10346 (1979). ~' G. A. Scheele and A. Haymovits, J. Biol. Chem. 255, 4918 (1980). ,2 A. Haymovits and G. A. Scheele, Proc. Natl. Acad. Sci. A. 73, 156 (1976). 28 SPECIALIZED METHODS [3] protein discharge; (b) defined the role of intracellular and extracellular calcium during stimulus-secretion coupling10; (c) identified changes in cellular cyclic nucleotide levels during cholinergic and hormonal stimulation~2; and (d) outlined mechanisms for the appearance of both parallel and nonparallel discharge of secretory proteins in the exocrine pancreas.

7, 139 (1978). ~3p. Novick, C. Field, and R. Schekman, Cell 21,205 (1980). 14L. A. Hunt and D. F. Summers, J. Virol. 20, 646 (1976). 15 F. N. Katz and H. F. Lodish, J. CellBiol. 80, 416 (1979). 16 D. M. Knipe, D. Baltimore, and H. F. Lodish, J. ViroL 21, 1128 (1977). ~7D. M. Knipe, H. F. Lodish, and D. Baltimore, J. Virol. 21, 1121 (1977). ~8S. Kornfeld, E. Li, and I. Tabas, J. Biol. Chem. 253, 7771 (1978). ~9C. L. Reading, E. E. Penhoet, and C. E. Ballou, J. Biol. Chem. 253, 5600 (1978). 20 p.

Rothman, Proc. Natl. Acad. Sci. A. 77, 3870(1980). 27j. E. Rothman and E. Fries, J. CellBiol. 89, 162 (1981). z8E. Fries and J. E. Rothman, J. CellBiol. 90, 697 (1981). 29W. G. Dunphy, E. Fries, L. J. Urbani, and J. E. Rothman, Proc. Natl. Acad. Sci. A. 78, 7453 (1981). 30A. L. Tarentino and F. Maley,Z Biol. Chem. 249, 811 (1974). 40 SPECIALIZED METHODS [4] plete growth medium). The CHO cell mutant clone 15B (obtained from S. Kornfeld, Washington University, St. Louis, Missouri zS) was grown in monolayers in the same medium.

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