Complex Carbohydrates Part B by Victor Ginsburg (Eds.)

By Victor Ginsburg (Eds.)

The severely acclaimed laboratory average, Methods in Enzymology, is without doubt one of the so much hugely revered courses within the box of biochemistry. when you consider that 1955, every one quantity has been eagerly awaited, usually consulted, and praised by means of researchers and reviewers alike. The sequence comprises a lot fabric nonetheless suitable at the present time - actually a necessary book for researchers in all fields of lifestyles sciences

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246, 3686 (1971). v R. C. Hughes and R. W. Jeanloz, Biochemistry 3, 1543 (1964). -T. Li, J. Biol. Chem. 241, 1010 (1966). ~ A. Tarentino, T. H. , and F. Maley, J. Biol. Chem. 245, 4150 (1970). J. Scocca and Y. C. Lee, J. Biol. Chem. 244, 4852 (1969). c~T. Muramatsu and F. Egami, J. Biochem. 62, 700 (1967). ~d y . Yasuda, N. Takahashi, and T. Murachi, Biochemistry 9, 25 (1970). •" T. Sukeno, A. L. Tarentino, T. H. , and F. Maley, Biochem. Biophys. Res. Commun. 45, 219 (1971). /J B. Weissmann and D.

The N - a c e t y l a t i o n reaction is not specific and will acetylate other free amino groups. If samples contain protein, it is necessary to isolate the hexosamine fraction by the method of Boas 6 prior to Nacetylation. T r e a t m e n t of the N - a c e t y l a t e d samples with a mixed-bed ion-exchange resin should remove any remaining amino acids. The quantitative determination of glucosamine, mannosamine, and galactosamine is possible with this procedure, but electrophoresis for a longer period of time is required for adequate separation.

Biol. Chem. 245, 2536 (1970). z R. G. Spiro and V. D. Bhoyroo, unpublished observations (1971). "* K. M. L. Agrawal and O. P. Bahl, J. Biol. Chem. 243, 103 (1968). n O. P. Bahl, J. Biol. Chem. 244, 575 (1969). ° P. V. Wagh, ][. Bornstein, and R. J. Winzler, J. Biol. Chem. 244, 658 (1969). O. P. Bahl and K. M. L. Agrawal, J. Biol. Chem. 244, 2970 (1969). S. Chipowsky and E. J. McGuire, Fed. , Fed. Amer. Soc. Exp. Biol. 28, 606 (1969). r L. Muir and Y. C. Lee, J. Biol. Chem. 245, 502 (1970). • R.

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