A History of Biochemistry: Selected Topics in the History of by Albert Neuberger, L. L. M. Van Deenen, Giorgio Semenza

By Albert Neuberger, L. L. M. Van Deenen, Giorgio Semenza

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For this reason DNA-RNA competitionhybridization experiments were undertaken by Grasso [184] in this laboratory and by Salser et al. [185] to study the synthesis of early T4 mRNAs under a variety of conditions, for example, during inhibition of protein synthesis. When chloramphenicol, an agent that effectively inhibits protein synthesis in prokaryotes, is included at the time of infection of E. coli B with T4 bacteriophage, a class of mRNAs is formed that may represent up to 30% of the total early species.

This infant discipline was then just forming from a confluence of ideas and technology originating in biophysics, biochemistry, microbiology, virology and genetics. Upon arriving in Philadelphia after my sabbatical leave in Sweden, I was invited to join the Medical Fellowship Board, the first panel of the National Science Foundation, and a subcommittee of Blood and Related Substances of the National Research Council. Through these extracurricular activities I made the acquaintance of many who became life-long friends and in some cases advisors on several such occasions.

Frere and Schroeder [132] recognized that the enzyme undergoes polymerization by oxidation of nonessential cysteinyl residues but without loss of activity. , by a partially compulsory order mechanism). The importance of the experiments on the reactions of azaserine and DON on FGAR amidotransferase is that they were the first clue to the existence of this type of irreversible active-site-directed reagents. Although these compounds as well as L-albizziin are of natural origin, other active-site-directed compounds have been devised and synthesized to perform specific inhibitions of enzyme activities.

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